In this directory there is the material used for the submitted paper: Title: DDGun: an untrained method for the prediction of protein stability changes upon single and multiple point variations Authors: Ludovica Montanucci1, Emidio Capriotti2*, Yotam Frank3, Nir Ben-Tal3, Piero Fariselli1* 1 Department of Comparative Biomedicine and Food Science (BCA), University of Padova, Legnaro, Italy. 2 BioFolD Unit, Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, Bologna, Italy. 3 Department of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel. * Corresponding Authors: Piero Fariselli, Emidio Capriotti. In the models.tar.gz file there are the models used for the predictions. In the predictions.tar.gz file there are the delta-delta-G predcitions through DDGun and DDGun3D methods.