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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
247 N V 0.71 7.0 25
247 N L -0.12 7.0 25
247 N I 1.02 7.0 25
247 N M -0.38 7.0 25
247 N F 0.02 7.0 25
247 N W -0.30 7.0 25
247 N Y -0.46 7.0 25
247 N G -1.96 7.0 25
247 N A -1.57 7.0 25
247 N P -0.44 7.0 25
247 N S -2.00 7.0 25
247 N T -1.89 7.0 25
247 N C -1.40 7.0 25
247 N H -2.23 7.0 25
247 N R -0.77 7.0 25
247 N K -1.18 7.0 25
247 N Q -1.29 7.0 25
247 N E -0.52 7.0 25
247 N D -1.54 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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