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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
313 H V -0.24 7.5 50
313 H L 0.41 7.5 50
313 H I -0.12 7.5 50
313 H M -0.04 7.5 50
313 H F 0.34 7.5 50
313 H W 0.15 7.5 50
313 H Y 0.38 7.5 50
313 H G -0.97 7.5 50
313 H A -0.58 7.5 50
313 H P -0.78 7.5 50
313 H S -1.05 7.5 50
313 H T -0.53 7.5 50
313 H C 0.66 7.5 50
313 H R -0.29 7.5 50
313 H K -0.78 7.5 50
313 H Q -0.92 7.5 50
313 H E -0.35 7.5 50
313 H N -2.25 7.5 50
313 H D -0.99 7.5 50
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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