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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
351 Q V 1.15 7.5 50
351 Q L 1.11 7.5 50
351 Q I 0.92 7.5 50
351 Q M -0.80 7.5 50
351 Q F -0.03 7.5 50
351 Q W -0.23 7.5 50
351 Q Y 0.04 7.5 50
351 Q G -1.43 7.5 50
351 Q A -0.52 7.5 50
351 Q P -1.01 7.5 50
351 Q S -0.94 7.5 50
351 Q T -1.01 7.5 50
351 Q C 1.09 7.5 50
351 Q H -0.79 7.5 50
351 Q R -0.35 7.5 50
351 Q K -0.29 7.5 50
351 Q E 0.17 7.5 50
351 Q N -1.77 7.5 50
351 Q D -0.69 7.5 50
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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