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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
270 A V 0.12 7.0 25
270 A L 0.47 7.0 25
270 A I 0.04 7.0 25
270 A M -0.30 7.0 25
270 A F 0.25 7.0 25
270 A W -0.28 7.0 25
270 A Y -0.30 7.0 25
270 A G -1.26 7.0 25
270 A P -1.19 7.0 25
270 A S -0.55 7.0 25
270 A T -0.51 7.0 25
270 A C -0.76 7.0 25
270 A H -0.51 7.0 25
270 A R -0.42 7.0 25
270 A K -0.66 7.0 25
270 A Q -0.73 7.0 25
270 A E -0.36 7.0 25
270 A N -0.62 7.0 25
270 A D -0.16 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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