I-Mutant2.0

Last Update 27/12/06



        
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**                                                                       **
**                             I-Mutant v2.0                             **
**         Predictor of Protein Stability Changes upon Mutations         **
**                                                                       **
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      SEQ File: fileseq.txt

      Position   WT  NEW     DDG    pH    T
           271    V    L   -0.45   7.0   25
           271    V    I   -1.01   7.0   25
           271    V    M   -1.81   7.0   25
           271    V    F   -1.90   7.0   25
           271    V    W   -1.50   7.0   25
           271    V    Y   -1.79   7.0   25
           271    V    G   -4.25   7.0   25
           271    V    A   -2.05   7.0   25
           271    V    P   -3.02   7.0   25
           271    V    S   -2.78   7.0   25
           271    V    T   -1.50   7.0   25
           271    V    C   -1.28   7.0   25
           271    V    H   -1.49   7.0   25
           271    V    R   -2.47   7.0   25
           271    V    K   -2.42   7.0   25
           271    V    Q   -2.14   7.0   25
           271    V    E   -1.38   7.0   25
           271    V    N   -2.99   7.0   25
           271    V    D   -1.52   7.0   25


      WT:  Aminoacid in Wild-Type Protein
      NEW: New Aminoacid after Mutation
      DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
             DDG<0: Decrease Stability
             DDG>0: Increase Stability
      T:   Temperature in Celsius degrees
      pH:  -log[H+]



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*                                                                         *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting  *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310.                                         *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
*                                                                         *
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