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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
2 T V 1.71 7.0 25
2 T L 1.41 7.0 25
2 T I 1.59 7.0 25
2 T M 1.47 7.0 25
2 T F 1.14 7.0 25
2 T W 1.01 7.0 25
2 T Y 2.44 7.0 25
2 T G -0.08 7.0 25
2 T A 0.30 7.0 25
2 T P 0.22 7.0 25
2 T S 1.33 7.0 25
2 T C 1.46 7.0 25
2 T H 1.07 7.0 25
2 T R 1.25 7.0 25
2 T K 0.86 7.0 25
2 T Q 0.80 7.0 25
2 T E 1.19 7.0 25
2 T N 1.39 7.0 25
2 T D 0.40 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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