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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
272 G V -0.61 7.0 25
272 G L -0.18 7.0 25
272 G I -0.62 7.0 25
272 G M -0.57 7.0 25
272 G F -0.47 7.0 25
272 G W -0.74 7.0 25
272 G Y -0.89 7.0 25
272 G A -1.45 7.0 25
272 G P -1.26 7.0 25
272 G S -1.48 7.0 25
272 G T -1.23 7.0 25
272 G C -0.85 7.0 25
272 G H -0.94 7.0 25
272 G R -1.22 7.0 25
272 G K -1.21 7.0 25
272 G Q -0.73 7.0 25
272 G E 0.08 7.0 25
272 G N -0.65 7.0 25
272 G D -0.99 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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