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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
273 V L -1.04 7.0 25
273 V I -1.29 7.0 25
273 V M -2.03 7.0 25
273 V F -2.06 7.0 25
273 V W -1.98 7.0 25
273 V Y -2.08 7.0 25
273 V G -4.18 7.0 25
273 V A -2.26 7.0 25
273 V P -3.16 7.0 25
273 V S -3.32 7.0 25
273 V T -1.79 7.0 25
273 V C -1.65 7.0 25
273 V H -1.62 7.0 25
273 V R -2.67 7.0 25
273 V K -2.54 7.0 25
273 V Q -2.40 7.0 25
273 V E -1.71 7.0 25
273 V N -3.37 7.0 25
273 V D -2.04 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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