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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
243 R V 0.55 5.0 60
243 R L -0.35 5.0 60
243 R I 0.75 5.0 60
243 R M -0.19 5.0 60
243 R F 0.34 5.0 60
243 R W 0.31 5.0 60
243 R Y 0.08 5.0 60
243 R G 0.73 5.0 60
243 R A 0.22 5.0 60
243 R P -0.30 5.0 60
243 R S -1.93 5.0 60
243 R T -0.57 5.0 60
243 R C 0.07 5.0 60
243 R H -0.46 5.0 60
243 R K -0.33 5.0 60
243 R Q -0.02 5.0 60
243 R E 0.23 5.0 60
243 R N -0.70 5.0 60
243 R D -0.30 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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