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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
309 I V -0.79 7.0 25
309 I L -0.18 7.0 25
309 I M -1.32 7.0 25
309 I F -1.61 7.0 25
309 I W -2.31 7.0 25
309 I Y -2.49 7.0 25
309 I G -3.20 7.0 25
309 I A -2.56 7.0 25
309 I P -2.00 7.0 25
309 I S -2.08 7.0 25
309 I T -2.20 7.0 25
309 I C -2.57 7.0 25
309 I H -1.89 7.0 25
309 I R -1.87 7.0 25
309 I K -2.14 7.0 25
309 I Q -2.06 7.0 25
309 I E -1.33 7.0 25
309 I N -1.40 7.0 25
309 I D -1.83 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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