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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
7 P V -0.05 7.0 25
7 P L 0.90 7.0 25
7 P I 0.10 7.0 25
7 P M -0.19 7.0 25
7 P F 0.31 7.0 25
7 P W -0.47 7.0 25
7 P Y -0.50 7.0 25
7 P G -1.83 7.0 25
7 P A -0.75 7.0 25
7 P S -0.74 7.0 25
7 P T -1.17 7.0 25
7 P C 0.34 7.0 25
7 P H -0.79 7.0 25
7 P R 0.54 7.0 25
7 P K -0.43 7.0 25
7 P Q -1.05 7.0 25
7 P E -0.34 7.0 25
7 P N -1.07 7.0 25
7 P D -1.07 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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