I-Mutant2.0

Last Update 27/12/06



        
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**                                                                       **
**                             I-Mutant v2.0                             **
**         Predictor of Protein Stability Changes upon Mutations         **
**                                                                       **
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      SEQ File: fileseq.txt

      Position   WT  NEW     DDG    pH    T
           311    M    V   -0.96   7.0   25
           311    M    L   -1.48   7.0   25
           311    M    I   -0.64   7.0   25
           311    M    F   -0.71   7.0   25
           311    M    W   -0.91   7.0   25
           311    M    Y   -0.82   7.0   25
           311    M    G   -1.59   7.0   25
           311    M    A   -1.37   7.0   25
           311    M    P   -1.06   7.0   25
           311    M    S   -0.74   7.0   25
           311    M    T   -0.87   7.0   25
           311    M    C   -0.58   7.0   25
           311    M    H   -0.92   7.0   25
           311    M    R   -1.47   7.0   25
           311    M    K   -1.40   7.0   25
           311    M    Q   -1.33   7.0   25
           311    M    E   -0.29   7.0   25
           311    M    N   -1.63   7.0   25
           311    M    D    0.26   7.0   25


      WT:  Aminoacid in Wild-Type Protein
      NEW: New Aminoacid after Mutation
      DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
             DDG<0: Decrease Stability
             DDG>0: Increase Stability
      T:   Temperature in Celsius degrees
      pH:  -log[H+]



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*                                                                         *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting  *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310.                                         *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
*                                                                         *
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