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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
312 K V -1.08 7.0 25
312 K L -1.20 7.0 25
312 K I -0.77 7.0 25
312 K M -0.74 7.0 25
312 K F -0.52 7.0 25
312 K W -1.63 7.0 25
312 K Y -0.90 7.0 25
312 K G -1.09 7.0 25
312 K A -0.92 7.0 25
312 K P -0.59 7.0 25
312 K S -0.87 7.0 25
312 K T -1.53 7.0 25
312 K C -0.91 7.0 25
312 K H -1.22 7.0 25
312 K R -1.72 7.0 25
312 K Q -1.65 7.0 25
312 K E -1.15 7.0 25
312 K N -1.78 7.0 25
312 K D -1.07 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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