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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
313 S V -0.61 7.0 25
313 S L -1.20 7.0 25
313 S I -0.33 7.0 25
313 S M -0.49 7.0 25
313 S F -0.63 7.0 25
313 S W -0.90 7.0 25
313 S Y -0.89 7.0 25
313 S G -1.20 7.0 25
313 S A -1.00 7.0 25
313 S P -1.49 7.0 25
313 S T -0.93 7.0 25
313 S C -1.90 7.0 25
313 S H -0.75 7.0 25
313 S R -1.25 7.0 25
313 S K -0.37 7.0 25
313 S Q -1.00 7.0 25
313 S E -0.58 7.0 25
313 S N -0.97 7.0 25
313 S D -0.12 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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