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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
314 I V -1.39 7.0 25
314 I L -0.29 7.0 25
314 I M -1.29 7.0 25
314 I F -1.64 7.0 25
314 I W -2.63 7.0 25
314 I Y -2.90 7.0 25
314 I G -3.46 7.0 25
314 I A -2.94 7.0 25
314 I P -2.66 7.0 25
314 I S -2.51 7.0 25
314 I T -2.30 7.0 25
314 I C -3.00 7.0 25
314 I H -2.22 7.0 25
314 I R -2.31 7.0 25
314 I K -2.31 7.0 25
314 I Q -2.42 7.0 25
314 I E -1.60 7.0 25
314 I N -1.33 7.0 25
314 I D -2.21 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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