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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
346 S V 1.83 7.0 25
346 S L 2.22 7.0 25
346 S I 1.65 7.0 25
346 S M 1.27 7.0 25
346 S F 0.68 7.0 25
346 S W 1.20 7.0 25
346 S Y 0.62 7.0 25
346 S G -0.25 7.0 25
346 S A 1.37 7.0 25
346 S P 0.92 7.0 25
346 S T 0.82 7.0 25
346 S C 0.99 7.0 25
346 S H 1.48 7.0 25
346 S R 0.48 7.0 25
346 S K 0.95 7.0 25
346 S Q 1.20 7.0 25
346 S E 1.30 7.0 25
346 S N 1.48 7.0 25
346 S D 1.16 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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