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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
248 L V -1.25 7.0 25
248 L I -1.11 7.0 25
248 L M -1.71 7.0 25
248 L F -0.74 7.0 25
248 L W -0.50 7.0 25
248 L Y -0.89 7.0 25
248 L G -4.86 7.0 25
248 L A -4.02 7.0 25
248 L P -1.48 7.0 25
248 L S -4.45 7.0 25
248 L T -4.79 7.0 25
248 L C -4.05 7.0 25
248 L H -2.23 7.0 25
248 L R -2.53 7.0 25
248 L K -2.87 7.0 25
248 L Q -2.38 7.0 25
248 L E -1.87 7.0 25
248 L N -3.05 7.0 25
248 L D -3.65 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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