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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
319 G V -1.16 7.0 25
319 G L -1.28 7.0 25
319 G I -0.52 7.0 25
319 G M -0.97 7.0 25
319 G F -1.08 7.0 25
319 G W -1.20 7.0 25
319 G Y -1.66 7.0 25
319 G A -0.25 7.0 25
319 G P -1.11 7.0 25
319 G S -1.44 7.0 25
319 G T -1.56 7.0 25
319 G C -1.34 7.0 25
319 G H -2.11 7.0 25
319 G R -1.61 7.0 25
319 G K -1.12 7.0 25
319 G Q -1.26 7.0 25
319 G E 0.34 7.0 25
319 G N -1.64 7.0 25
319 G D -1.03 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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