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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
320 I V -0.75 7.0 25
320 I L -0.82 7.0 25
320 I M -1.91 7.0 25
320 I F -1.76 7.0 25
320 I W -2.78 7.0 25
320 I Y -3.24 7.0 25
320 I G -3.63 7.0 25
320 I A -3.27 7.0 25
320 I P -2.74 7.0 25
320 I S -3.01 7.0 25
320 I T -2.65 7.0 25
320 I C -3.83 7.0 25
320 I H -2.99 7.0 25
320 I R -2.53 7.0 25
320 I K -2.27 7.0 25
320 I Q -2.76 7.0 25
320 I E -1.62 7.0 25
320 I N -1.64 7.0 25
320 I D -2.59 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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