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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
18 T V -0.40 5.0 60
18 T L -0.80 5.0 60
18 T I -0.19 5.0 60
18 T M -0.34 5.0 60
18 T F 0.56 5.0 60
18 T W 0.23 5.0 60
18 T Y 1.25 5.0 60
18 T G -2.07 5.0 60
18 T A -0.92 5.0 60
18 T P -1.34 5.0 60
18 T S -0.72 5.0 60
18 T C -0.75 5.0 60
18 T H -1.68 5.0 60
18 T R -0.05 5.0 60
18 T K -0.74 5.0 60
18 T Q -1.44 5.0 60
18 T E -0.10 5.0 60
18 T N -1.08 5.0 60
18 T D -0.46 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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