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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
321 D V -0.51 7.0 25
321 D L -1.19 7.0 25
321 D I -0.69 7.0 25
321 D M -0.92 7.0 25
321 D F -0.85 7.0 25
321 D W -0.87 7.0 25
321 D Y -0.87 7.0 25
321 D G -0.79 7.0 25
321 D A -0.57 7.0 25
321 D P -1.14 7.0 25
321 D S -0.24 7.0 25
321 D T -0.68 7.0 25
321 D C -1.52 7.0 25
321 D H -1.04 7.0 25
321 D R -1.01 7.0 25
321 D K -0.41 7.0 25
321 D Q -0.68 7.0 25
321 D E 0.28 7.0 25
321 D N -1.23 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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