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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
322 T V -0.53 7.0 25
322 T L -1.54 7.0 25
322 T I -0.80 7.0 25
322 T M -0.71 7.0 25
322 T F -0.66 7.0 25
322 T W -0.56 7.0 25
322 T Y -0.06 7.0 25
322 T G -1.12 7.0 25
322 T A -1.18 7.0 25
322 T P -1.60 7.0 25
322 T S -0.70 7.0 25
322 T C -1.14 7.0 25
322 T H -1.88 7.0 25
322 T R -0.35 7.0 25
322 T K -0.66 7.0 25
322 T Q -1.84 7.0 25
322 T E -0.04 7.0 25
322 T N -1.49 7.0 25
322 T D 0.17 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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