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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
323 V L -2.83 7.0 25
323 V I -1.18 7.0 25
323 V M -2.68 7.0 25
323 V F -3.78 7.0 25
323 V W -2.69 7.0 25
323 V Y -3.13 7.0 25
323 V G -3.72 7.0 25
323 V A -2.25 7.0 25
323 V P -3.21 7.0 25
323 V S -3.37 7.0 25
323 V T -2.90 7.0 25
323 V C -1.99 7.0 25
323 V H -3.11 7.0 25
323 V R -3.09 7.0 25
323 V K -2.54 7.0 25
323 V Q -2.88 7.0 25
323 V E -2.25 7.0 25
323 V N -4.12 7.0 25
323 V D -2.56 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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