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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
324 V L -2.87 7.0 25
324 V I -1.13 7.0 25
324 V M -2.82 7.0 25
324 V F -3.74 7.0 25
324 V W -2.60 7.0 25
324 V Y -3.08 7.0 25
324 V G -3.99 7.0 25
324 V A -2.55 7.0 25
324 V P -3.37 7.0 25
324 V S -3.59 7.0 25
324 V T -3.17 7.0 25
324 V C -2.09 7.0 25
324 V H -3.26 7.0 25
324 V R -3.14 7.0 25
324 V K -2.67 7.0 25
324 V Q -2.92 7.0 25
324 V E -2.30 7.0 25
324 V N -4.01 7.0 25
324 V D -2.71 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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