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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
325 D V -0.83 7.0 25
325 D L -1.44 7.0 25
325 D I -1.06 7.0 25
325 D M -1.02 7.0 25
325 D F -0.92 7.0 25
325 D W -1.03 7.0 25
325 D Y -1.04 7.0 25
325 D G -0.70 7.0 25
325 D A -0.93 7.0 25
325 D P -1.72 7.0 25
325 D S -0.92 7.0 25
325 D T -1.33 7.0 25
325 D C -1.65 7.0 25
325 D H -1.35 7.0 25
325 D R -1.07 7.0 25
325 D K -0.50 7.0 25
325 D Q -0.85 7.0 25
325 D E -0.18 7.0 25
325 D N -1.27 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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