I-Mutant2.0

Last Update 27/12/06



        
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**                                                                       **
**                             I-Mutant v2.0                             **
**         Predictor of Protein Stability Changes upon Mutations         **
**                                                                       **
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      SEQ File: fileseq.txt

      Position   WT  NEW     DDG    pH    T
           326    L    V   -1.65   7.0   25
           326    L    I   -1.47   7.0   25
           326    L    M   -1.70   7.0   25
           326    L    F   -1.16   7.0   25
           326    L    W   -0.85   7.0   25
           326    L    Y   -1.44   7.0   25
           326    L    G   -3.83   7.0   25
           326    L    A   -3.95   7.0   25
           326    L    P   -2.08   7.0   25
           326    L    S   -3.73   7.0   25
           326    L    T   -4.10   7.0   25
           326    L    C   -3.90   7.0   25
           326    L    H   -2.37   7.0   25
           326    L    R   -2.52   7.0   25
           326    L    K   -2.60   7.0   25
           326    L    Q   -2.60   7.0   25
           326    L    E   -1.52   7.0   25
           326    L    N   -3.25   7.0   25
           326    L    D   -3.43   7.0   25


      WT:  Aminoacid in Wild-Type Protein
      NEW: New Aminoacid after Mutation
      DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
             DDG<0: Decrease Stability
             DDG>0: Increase Stability
      T:   Temperature in Celsius degrees
      pH:  -log[H+]



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*                                                                         *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting  *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310.                                         *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
*                                                                         *
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