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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
327 P V -0.73 7.0 25
327 P L -1.48 7.0 25
327 P I -0.65 7.0 25
327 P M -0.91 7.0 25
327 P F -0.68 7.0 25
327 P W -1.14 7.0 25
327 P Y -1.73 7.0 25
327 P G -0.79 7.0 25
327 P A -1.69 7.0 25
327 P S -1.55 7.0 25
327 P T -1.35 7.0 25
327 P C -1.63 7.0 25
327 P H -1.49 7.0 25
327 P R -1.15 7.0 25
327 P K -0.92 7.0 25
327 P Q -1.47 7.0 25
327 P E -0.54 7.0 25
327 P N -1.66 7.0 25
327 P D -1.34 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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