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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
328 V L -1.97 7.0 25
328 V I -1.16 7.0 25
328 V M -2.46 7.0 25
328 V F -2.89 7.0 25
328 V W -2.13 7.0 25
328 V Y -2.80 7.0 25
328 V G -3.62 7.0 25
328 V A -2.74 7.0 25
328 V P -3.65 7.0 25
328 V S -3.37 7.0 25
328 V T -2.40 7.0 25
328 V C -1.80 7.0 25
328 V H -2.58 7.0 25
328 V R -3.14 7.0 25
328 V K -2.61 7.0 25
328 V Q -2.74 7.0 25
328 V E -1.80 7.0 25
328 V N -3.92 7.0 25
328 V D -2.44 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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