I-Mutant2.0

Last Update 27/12/06



        
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**                                                                       **
**                             I-Mutant v2.0                             **
**         Predictor of Protein Stability Changes upon Mutations         **
**                                                                       **
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      SEQ File: fileseq.txt

      Position   WT  NEW     DDG    pH    T
           329    G    V   -0.68   7.0   25
           329    G    L   -1.17   7.0   25
           329    G    I   -0.78   7.0   25
           329    G    M   -0.95   7.0   25
           329    G    F   -1.16   7.0   25
           329    G    W   -1.14   7.0   25
           329    G    Y   -1.73   7.0   25
           329    G    A   -1.20   7.0   25
           329    G    P   -1.45   7.0   25
           329    G    S   -1.98   7.0   25
           329    G    T   -1.65   7.0   25
           329    G    C   -1.40   7.0   25
           329    G    H   -1.89   7.0   25
           329    G    R   -1.71   7.0   25
           329    G    K   -1.30   7.0   25
           329    G    Q   -1.11   7.0   25
           329    G    E   -0.13   7.0   25
           329    G    N   -1.11   7.0   25
           329    G    D   -1.52   7.0   25


      WT:  Aminoacid in Wild-Type Protein
      NEW: New Aminoacid after Mutation
      DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
             DDG<0: Decrease Stability
             DDG>0: Increase Stability
      T:   Temperature in Celsius degrees
      pH:  -log[H+]



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*                                                                         *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting  *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310.                                         *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
*                                                                         *
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