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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
249 Q V 0.11 7.0 25
249 Q L -0.71 7.0 25
249 Q I 0.09 7.0 25
249 Q M -1.69 7.0 25
249 Q F 0.03 7.0 25
249 Q W -0.41 7.0 25
249 Q Y -0.25 7.0 25
249 Q G -1.76 7.0 25
249 Q A -1.22 7.0 25
249 Q P -1.22 7.0 25
249 Q S -2.45 7.0 25
249 Q T -1.83 7.0 25
249 Q C -0.76 7.0 25
249 Q H -1.53 7.0 25
249 Q R -1.49 7.0 25
249 Q K -0.65 7.0 25
249 Q E -0.19 7.0 25
249 Q N -2.20 7.0 25
249 Q D -1.00 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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