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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
330 L V -0.85 7.0 25
330 L I -1.09 7.0 25
330 L M -1.20 7.0 25
330 L F -0.63 7.0 25
330 L W -0.63 7.0 25
330 L Y -1.06 7.0 25
330 L G -3.74 7.0 25
330 L A -3.77 7.0 25
330 L P -1.75 7.0 25
330 L S -3.33 7.0 25
330 L T -3.28 7.0 25
330 L C -3.23 7.0 25
330 L H -1.66 7.0 25
330 L R -2.18 7.0 25
330 L K -2.30 7.0 25
330 L Q -2.03 7.0 25
330 L E -1.36 7.0 25
330 L N -2.73 7.0 25
330 L D -3.59 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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