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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
174 T V -0.24 5.0 60
174 T L -0.51 5.0 60
174 T I -0.93 5.0 60
174 T M 0.10 5.0 60
174 T F 0.10 5.0 60
174 T W 0.03 5.0 60
174 T Y 0.73 5.0 60
174 T G -0.59 5.0 60
174 T A -0.44 5.0 60
174 T P -1.50 5.0 60
174 T S -0.31 5.0 60
174 T C 0.22 5.0 60
174 T H -0.51 5.0 60
174 T R 0.20 5.0 60
174 T K -0.39 5.0 60
174 T Q -1.13 5.0 60
174 T E 0.24 5.0 60
174 T N -0.08 5.0 60
174 T D -0.20 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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