I-Mutant2.0

Last Update 27/12/06



        
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**                                                                       **
**                             I-Mutant v2.0                             **
**         Predictor of Protein Stability Changes upon Mutations         **
**                                                                       **
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      SEQ File: fileseq.txt

      Position   WT  NEW     DDG    pH    T
           174    T    V   -0.24   5.0   60
           174    T    L   -0.51   5.0   60
           174    T    I   -0.93   5.0   60
           174    T    M    0.10   5.0   60
           174    T    F    0.10   5.0   60
           174    T    W    0.03   5.0   60
           174    T    Y    0.73   5.0   60
           174    T    G   -0.59   5.0   60
           174    T    A   -0.44   5.0   60
           174    T    P   -1.50   5.0   60
           174    T    S   -0.31   5.0   60
           174    T    C    0.22   5.0   60
           174    T    H   -0.51   5.0   60
           174    T    R    0.20   5.0   60
           174    T    K   -0.39   5.0   60
           174    T    Q   -1.13   5.0   60
           174    T    E    0.24   5.0   60
           174    T    N   -0.08   5.0   60
           174    T    D   -0.20   5.0   60


      WT:  Aminoacid in Wild-Type Protein
      NEW: New Aminoacid after Mutation
      DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
             DDG<0: Decrease Stability
             DDG>0: Increase Stability
      T:   Temperature in Celsius degrees
      pH:  -log[H+]



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*                                                                         *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting  *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310.                                         *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
*                                                                         *
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