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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
331 N V 2.07 7.0 25
331 N L 1.68 7.0 25
331 N I 2.06 7.0 25
331 N M 1.11 7.0 25
331 N F 1.17 7.0 25
331 N W 0.93 7.0 25
331 N Y 0.58 7.0 25
331 N G -0.38 7.0 25
331 N A 0.10 7.0 25
331 N P 0.54 7.0 25
331 N S -0.27 7.0 25
331 N T 0.66 7.0 25
331 N C 0.09 7.0 25
331 N H -0.50 7.0 25
331 N R 0.06 7.0 25
331 N K 0.43 7.0 25
331 N Q 0.05 7.0 25
331 N E 1.08 7.0 25
331 N D -0.16 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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