I-Mutant2.0

Last Update 27/12/06



        
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**                                                                       **
**                             I-Mutant v2.0                             **
**         Predictor of Protein Stability Changes upon Mutations         **
**                                                                       **
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      SEQ File: fileseq.txt

      Position   WT  NEW     DDG    pH    T
           175    V    L   -0.86   5.0   60
           175    V    I   -0.71   5.0   60
           175    V    M   -1.51   5.0   60
           175    V    F   -1.56   5.0   60
           175    V    W   -1.27   5.0   60
           175    V    Y   -1.66   5.0   60
           175    V    G   -2.66   5.0   60
           175    V    A   -1.17   5.0   60
           175    V    P   -2.32   5.0   60
           175    V    S   -2.30   5.0   60
           175    V    T   -1.14   5.0   60
           175    V    C   -0.52   5.0   60
           175    V    H   -1.04   5.0   60
           175    V    R   -1.72   5.0   60
           175    V    K   -1.68   5.0   60
           175    V    Q   -1.75   5.0   60
           175    V    E   -1.00   5.0   60
           175    V    N   -2.06   5.0   60
           175    V    D   -1.69   5.0   60


      WT:  Aminoacid in Wild-Type Protein
      NEW: New Aminoacid after Mutation
      DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
             DDG<0: Decrease Stability
             DDG>0: Increase Stability
      T:   Temperature in Celsius degrees
      pH:  -log[H+]



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*                                                                         *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting  *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310.                                         *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
*                                                                         *
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