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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
175 V L -0.86 5.0 60
175 V I -0.71 5.0 60
175 V M -1.51 5.0 60
175 V F -1.56 5.0 60
175 V W -1.27 5.0 60
175 V Y -1.66 5.0 60
175 V G -2.66 5.0 60
175 V A -1.17 5.0 60
175 V P -2.32 5.0 60
175 V S -2.30 5.0 60
175 V T -1.14 5.0 60
175 V C -0.52 5.0 60
175 V H -1.04 5.0 60
175 V R -1.72 5.0 60
175 V K -1.68 5.0 60
175 V Q -1.75 5.0 60
175 V E -1.00 5.0 60
175 V N -2.06 5.0 60
175 V D -1.69 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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