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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
332 L V -0.98 7.0 25
332 L I -1.35 7.0 25
332 L M -1.48 7.0 25
332 L F -0.72 7.0 25
332 L W -0.85 7.0 25
332 L Y -1.45 7.0 25
332 L G -4.56 7.0 25
332 L A -4.24 7.0 25
332 L P -1.72 7.0 25
332 L S -3.90 7.0 25
332 L T -3.77 7.0 25
332 L C -3.77 7.0 25
332 L H -2.37 7.0 25
332 L R -2.62 7.0 25
332 L K -2.77 7.0 25
332 L Q -2.46 7.0 25
332 L E -1.70 7.0 25
332 L N -3.07 7.0 25
332 L D -3.80 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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