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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
333 Q V 1.24 7.0 25
333 Q L 0.66 7.0 25
333 Q I 0.88 7.0 25
333 Q M -0.84 7.0 25
333 Q F 0.43 7.0 25
333 Q W 0.33 7.0 25
333 Q Y 0.16 7.0 25
333 Q G -0.63 7.0 25
333 Q A -0.27 7.0 25
333 Q P -0.49 7.0 25
333 Q S -0.91 7.0 25
333 Q T -0.32 7.0 25
333 Q C 0.37 7.0 25
333 Q H -0.54 7.0 25
333 Q R -0.74 7.0 25
333 Q K 0.19 7.0 25
333 Q E 0.83 7.0 25
333 Q N -1.65 7.0 25
333 Q D -0.11 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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