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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
334 D V 0.07 7.0 25
334 D L -0.60 7.0 25
334 D I -0.50 7.0 25
334 D M -0.92 7.0 25
334 D F -0.45 7.0 25
334 D W -0.45 7.0 25
334 D Y -0.66 7.0 25
334 D G -1.19 7.0 25
334 D A -0.77 7.0 25
334 D P -1.07 7.0 25
334 D S -0.80 7.0 25
334 D T -0.85 7.0 25
334 D C -1.09 7.0 25
334 D H -0.71 7.0 25
334 D R -0.79 7.0 25
334 D K -0.93 7.0 25
334 D Q -0.56 7.0 25
334 D E 0.03 7.0 25
334 D N -1.37 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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