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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
335 Q V 1.16 7.0 25
335 Q L 0.57 7.0 25
335 Q I 0.86 7.0 25
335 Q M -0.94 7.0 25
335 Q F 0.17 7.0 25
335 Q W 0.15 7.0 25
335 Q Y -0.04 7.0 25
335 Q G -0.67 7.0 25
335 Q A -0.39 7.0 25
335 Q P -0.39 7.0 25
335 Q S -0.89 7.0 25
335 Q T -0.44 7.0 25
335 Q C 0.40 7.0 25
335 Q H -0.56 7.0 25
335 Q R -0.84 7.0 25
335 Q K -0.18 7.0 25
335 Q E 0.68 7.0 25
335 Q N -1.97 7.0 25
335 Q D 0.00 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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