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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
20 H V 0.26 7.0 25
20 H L 1.15 7.0 25
20 H I 0.38 7.0 25
20 H M 0.18 7.0 25
20 H F -0.32 7.0 25
20 H W -0.23 7.0 25
20 H Y 0.54 7.0 25
20 H G -0.52 7.0 25
20 H A -0.12 7.0 25
20 H P -0.24 7.0 25
20 H S -0.31 7.0 25
20 H T -0.71 7.0 25
20 H C 0.65 7.0 25
20 H R -0.10 7.0 25
20 H K -0.19 7.0 25
20 H Q -0.17 7.0 25
20 H E 0.32 7.0 25
20 H N -0.65 7.0 25
20 H D -1.09 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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