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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
336 T V -0.01 7.0 25
336 T L -0.69 7.0 25
336 T I -0.72 7.0 25
336 T M -0.52 7.0 25
336 T F -0.08 7.0 25
336 T W -0.03 7.0 25
336 T Y 0.60 7.0 25
336 T G -1.64 7.0 25
336 T A -1.19 7.0 25
336 T P -1.49 7.0 25
336 T S -0.80 7.0 25
336 T C -0.62 7.0 25
336 T H -1.24 7.0 25
336 T R -0.43 7.0 25
336 T K -1.13 7.0 25
336 T Q -1.50 7.0 25
336 T E -0.20 7.0 25
336 T N -0.85 7.0 25
336 T D -0.06 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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