***************************************************************************
** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
***************************************************************************
SEQ File: fileseq.txt
Position WT NEW DDG pH T
26 Q V 0.52 7.0 55
26 Q L 0.76 7.0 55
26 Q I 0.93 7.0 55
26 Q M -0.71 7.0 55
26 Q F 0.08 7.0 55
26 Q W 0.11 7.0 55
26 Q Y 0.18 7.0 55
26 Q G -1.07 7.0 55
26 Q A 0.04 7.0 55
26 Q P -0.96 7.0 55
26 Q S -0.54 7.0 55
26 Q T -0.40 7.0 55
26 Q C 1.10 7.0 55
26 Q H -1.23 7.0 55
26 Q R -0.79 7.0 55
26 Q K -0.45 7.0 55
26 Q E 0.30 7.0 55
26 Q N -2.35 7.0 55
26 Q D -0.10 7.0 55
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
***************************************************************************
* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
***************************************************************************