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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
337 T V -0.25 7.0 25
337 T L -0.74 7.0 25
337 T I -0.85 7.0 25
337 T M -0.52 7.0 25
337 T F -0.25 7.0 25
337 T W -0.06 7.0 25
337 T Y 0.41 7.0 25
337 T G -2.04 7.0 25
337 T A -1.38 7.0 25
337 T P -1.53 7.0 25
337 T S -0.70 7.0 25
337 T C -0.70 7.0 25
337 T H -1.24 7.0 25
337 T R -0.61 7.0 25
337 T K -1.45 7.0 25
337 T Q -1.60 7.0 25
337 T E -0.35 7.0 25
337 T N -1.06 7.0 25
337 T D -0.03 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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