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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
250 V L -1.59 7.0 25
250 V I -0.76 7.0 25
250 V M -2.14 7.0 25
250 V F -2.23 7.0 25
250 V W -1.61 7.0 25
250 V Y -2.07 7.0 25
250 V G -4.36 7.0 25
250 V A -3.04 7.0 25
250 V P -2.95 7.0 25
250 V S -3.93 7.0 25
250 V T -2.71 7.0 25
250 V C -1.76 7.0 25
250 V H -2.79 7.0 25
250 V R -2.46 7.0 25
250 V K -2.44 7.0 25
250 V Q -2.44 7.0 25
250 V E -1.94 7.0 25
250 V N -2.93 7.0 25
250 V D -2.07 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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