I-Mutant2.0

Last Update 27/12/06



        
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**                                                                       **
**                             I-Mutant v2.0                             **
**         Predictor of Protein Stability Changes upon Mutations         **
**                                                                       **
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      SEQ File: fileseq.txt

      Position   WT  NEW     DDG    pH    T
           338    A    V    0.27   7.0   25
           338    A    L    0.16   7.0   25
           338    A    I    0.17   7.0   25
           338    A    M   -0.51   7.0   25
           338    A    F   -0.54   7.0   25
           338    A    W   -0.46   7.0   25
           338    A    Y   -1.07   7.0   25
           338    A    G   -1.26   7.0   25
           338    A    P   -0.81   7.0   25
           338    A    S   -0.46   7.0   25
           338    A    T   -0.83   7.0   25
           338    A    C   -0.56   7.0   25
           338    A    H   -0.55   7.0   25
           338    A    R   -0.48   7.0   25
           338    A    K   -1.32   7.0   25
           338    A    Q   -0.76   7.0   25
           338    A    E   -0.60   7.0   25
           338    A    N   -1.06   7.0   25
           338    A    D   -0.13   7.0   25


      WT:  Aminoacid in Wild-Type Protein
      NEW: New Aminoacid after Mutation
      DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
             DDG<0: Decrease Stability
             DDG>0: Increase Stability
      T:   Temperature in Celsius degrees
      pH:  -log[H+]



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*                                                                         *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting  *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310.                                         *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
*                                                                         *
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