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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
83 S V 0.63 7.0 25
83 S L -0.17 7.0 25
83 S I 0.58 7.0 25
83 S M 0.24 7.0 25
83 S F 0.79 7.0 25
83 S W 0.07 7.0 25
83 S Y -0.07 7.0 25
83 S G -1.43 7.0 25
83 S A -0.29 7.0 25
83 S P -1.05 7.0 25
83 S T -0.15 7.0 25
83 S C -1.23 7.0 25
83 S H -0.56 7.0 25
83 S R -0.21 7.0 25
83 S K 0.17 7.0 25
83 S Q -0.12 7.0 25
83 S E -0.12 7.0 25
83 S N 0.12 7.0 25
83 S D -0.33 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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