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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
6 A V 0.73 7.0 25
6 A L 1.00 7.0 25
6 A I 1.08 7.0 25
6 A M 0.86 7.0 25
6 A F 0.95 7.0 25
6 A W 0.12 7.0 25
6 A Y 0.05 7.0 25
6 A G -1.14 7.0 25
6 A P -0.19 7.0 25
6 A S 0.57 7.0 25
6 A T -0.31 7.0 25
6 A C 1.02 7.0 25
6 A H -0.50 7.0 25
6 A R 1.23 7.0 25
6 A K -0.13 7.0 25
6 A Q -0.31 7.0 25
6 A E -0.27 7.0 25
6 A N 0.01 7.0 25
6 A D -0.39 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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