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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
340 V L 0.19 7.0 25
340 V I -0.25 7.0 25
340 V M -1.53 7.0 25
340 V F -2.25 7.0 25
340 V W -1.24 7.0 25
340 V Y -1.87 7.0 25
340 V G -3.47 7.0 25
340 V A -1.90 7.0 25
340 V P -2.08 7.0 25
340 V S -2.24 7.0 25
340 V T -1.52 7.0 25
340 V C -0.45 7.0 25
340 V H -1.09 7.0 25
340 V R -2.10 7.0 25
340 V K -2.44 7.0 25
340 V Q -1.58 7.0 25
340 V E -0.98 7.0 25
340 V N -2.89 7.0 25
340 V D -1.31 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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