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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
400 E V 1.08 5.0 60
400 E L 0.41 5.0 60
400 E I 1.42 5.0 60
400 E M 0.93 5.0 60
400 E F 1.55 5.0 60
400 E W 0.35 5.0 60
400 E Y 0.39 5.0 60
400 E G -0.11 5.0 60
400 E A -0.16 5.0 60
400 E P -0.07 5.0 60
400 E S -0.61 5.0 60
400 E T 0.26 5.0 60
400 E C 0.33 5.0 60
400 E H -0.55 5.0 60
400 E R 0.62 5.0 60
400 E K -0.54 5.0 60
400 E Q -0.03 5.0 60
400 E N -0.86 5.0 60
400 E D -0.29 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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