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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
408 D V 0.22 5.0 60
408 D L 0.07 5.0 60
408 D I -0.06 5.0 60
408 D M 0.32 5.0 60
408 D F 0.00 5.0 60
408 D W -0.52 5.0 60
408 D Y 0.28 5.0 60
408 D G -0.12 5.0 60
408 D A -0.41 5.0 60
408 D P -1.34 5.0 60
408 D S -0.35 5.0 60
408 D T -1.18 5.0 60
408 D C -0.01 5.0 60
408 D H -0.51 5.0 60
408 D R 0.36 5.0 60
408 D K -0.46 5.0 60
408 D Q 0.39 5.0 60
408 D E -0.22 5.0 60
408 D N -0.35 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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